Portable enrichment, aliquoting, and testing device of microorganisms and toxins

A portable device for detecting biological hazard agents in a solid macrosample comprising:

• a container comprising an external surface provided with an outer thread, the container enclosing a first housing filled with a culture medium for biological hazard agents,
• a second housing for receiving the solid macrosample,
• a lid comprising an outer perimeter wall provided with an inner thread, said inner thread being complementary to the outer thread of the external surface of the container, so that the lid is adapted to be coupled by screwing along a coupling course to the container,
• a tearable first seal that separates the first housing and the second housing and that, when teared, communicates the first housing and the second housing such that the culture medium for biological hazard agents and the solid macrosample are brought into contact and mix with each other to obtain an enriched test sample mixture from the solid macrosample,
• a confined receptacle located within the container and communicates with the first housing or with the second housing through a filter or mesh to receive a predetermined fine filtered portion of the enriched test sample mixture therefrom,
• an immunochromatography strip enclosed within a third housing for sensing a presence of biological hazard agents in a predetermined aliquot of the predetermined fine filtered portion of the enriched test sample mixture, and
• said third housing being provided with a transparent surface that allows the immunochromatography strip to be seen from the outside, wherein the third housing is adapted to access said confined receptacle through an aperture sealed with a tearable second seal formed in between the confined receptacle and the third housing to extract said predetermined aliquot of the predetermined fine filtered portion of the enriched test sample mixture therefrom, to be accessed by the immunochromatography strip,
• wherein the second housing has an inlet surface greater than 10 cm2 and comprises at least one visual indicator corresponding to a predetermined filling volume,
• wherein the second housing is in the lid, wherein the lid is a plate,
• wherein the lid further comprises an inner perimeter wall arranged within the outer perimeter wall, the inner perimeter wall enclosing the second housing and having a brim and a plurality of cutting elements distributed on the brim, the plurality of cutting elements being configured to tear the tearable first seal along a circumferential path when the lid is screwed to the container from a first determined coupling position to reach a determined second coupling position along the coupling course.