Arrays and methods for guided cell patterning
AssigneeUniversity of Washington
InventorsMiqin Zhang, Mandana Veiseh
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Abstract
Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion.
Claims22
An array for guided cell patterning, comprising a plurality of individually immobilized cells isolated on an inert surface resistant to cell adhesion, wherein the inert surface comprises a silicon oxide surface having polyalkylene oxide moieties covalently coupled thereto, wherein the silicon oxide surface comprises from about 40% to 65% by weight Si, from about 5% to about 20% by weight SiOx<2, and from about 20% to about 40% by weight SiO2.
An array for guided cell patterning, comprising a plurality of individually immobilized cells isolated on an inert surface resistant to cell adhesion, wherein the inert surface comprises a silicon oxide surface having polyalkylene oxide moieties covalently coupled thereto, wherein each cell is immobilized through the interaction of one or more peptide ligands and one or more cell surface receptors of the immobilized cell, wherein the one or more peptide ligands is chlorotoxin.
An array for guided cell patterning, comprising a plurality of individually immobilized cells isolated on an inert surface resistant to cell adhesion, wherein the inert surface comprises a silicon oxide surface having polyalkylene oxide moieties covalently coupled thereto, wherein each cell is immobilized through the interaction of one or more peptide ligands and one or more cell surface receptors of the immobilized cell, wherein the one or more peptide ligands is nerve growth factor.
Dependencies are inferred from the claim text.