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US 10,119,172 B2Granted
Methods and reagents for detecting ebola virus
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsUte Stroeher, Tara Sealy, Jonathan Towner, Stuart Nichol, César Albariño
Life of this patent
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Abstract
Probes and primers are disclosed for detecting EBOV nucleic acid in a sample. Methods are also disclosed that utilize these probes and primers, wherein the methods can be used to detect an EBOV in a sample to identify a subject with an EBOV infection.
Claims24
A method for detecting an EBOV NP nucleic acid in a biological sample from a subject, comprising:
- amplifying a DNA template produced from a reverse transcription reaction performed on nucleic acid containing RNA isolated from the biological sample by:
- contacting the DNA template under conditions suitable for amplification with a set of forward and reverse primers that can be used to amplify an EBOV nucleic acid molecule comprising the sequence set forth as SEQ ID NO:
- 1, and
- performing a polymerase chain reaction amplification to form an amplified DNA product;
- and
- contacting the amplified DNA product with a probe that can hybridize to the amplified DNA product;
- wherein hybridization of the probe to the amplified DNA product indicates that EBOV NP nucleic acid is present in the sample.
4 more elements
A method for detecting an EBOV VP40 nucleic acid in a biological sample from a subject, comprising:
- amplifying a DNA template produced from a reverse transcription reaction performed on nucleic acid containing RNA isolated from the biological sample by:
- contacting the DNA template under conditions suitable for amplification with a set of forward and reverse primers that can be used to amplify an EBOV nucleic acid molecule comprising the sequence set forth as SEQ ID NO:
- 5, and
- performing a polymerase chain reaction amplification to form an amplified DNA product;
- and
- contacting the amplified DNA product with a probe that can hybridize to the amplified DNA product;
- wherein hybridization of the probe to the amplified DNA product indicates that EBOV NP nucleic acid is present in the sample.
4 more elements
Dependencies are inferred from the claim text.