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US 10,119,170 B2Granted
Detection of chromosomal region copy number changes to diagnose melanoma
AssigneeThe Regents of the University of California
InventorsBoris Bastian, Daniel Pinkel
Life of this patent
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Abstract
This invention provides methods of detecting melanoma. The methods comprises detecting a gain or loss of certain chromosomal regions that undergo copy number changes in melanoma.
Claims4
A method of detecting a gain in copy number of a chromosomal region 6p21 in a melanoma in a human patient that has a primary mucosal melanoma, the method comprising:
- contacting a DNA sample obtained from a biopsy of the primary mucosal melanoma with a probe that selectively hybridizes to the chromosomal region;
- and
- measuring the level of binding of the probe to the DNA sample obtained from the mucosal melanoma and to a control DNA sample from a healthy tissue.
A method of detecting a gain in copy number at a chromosomal region 6p21 in a melanoma in a patient that has a primary mucosal melanoma, the method comprising:
- performing a quantitative polymerase chain reaction on a DNA sample obtained from a biopsy of a primary mucosal melanoma arising on a mucosal membrane of the patient using primers that selectively amplify DNA within chromosomal region 6p21;
- and
- measuring the amount of amplified product obtained from the DNA sample obtained from the biopsy of the primary mucosal melanoma and from a normal control tissue.
Dependencies are inferred from the claim text.