Anthocyanins with specific properties
AssigneeEvolva SA
InventorsJorgen Hansen, Patricia Hoyle, Michael Naesby, Joseph M. Sheridan, Jonathan R. Heal
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Abstract
The present disclosure provides methods for identifying anthocyanins with improved stability, color, or hue using a screening method, and producing anthocyanins with improved stability, color, or hue in, for example, host cells comprising one or more heterologous glycosyltransferase nucleic acid molecules and one or more heterologous acyltransferase nucleic acid molecules.
Claims20
A method of identifying modified anthocyanins with improved stability, color, or hue using a screening method comprising the steps of:
- (a) providing one or more microbial host cells capable of producing at least one anthocyanidin, wherein said host cells comprise one or more heterologous glycosyltransferase (GT) nucleic acid molecules and one or more heterologous acyltransferase (AT) nucleic acid molecules;
- (b) cultivating said host cells under conditions allowing growth of said cells and production of at least one anthocyanin thereby;
- (c) screening said host cells and/or culture medium for modified anthocyanins with improved stability, color, or hue;
- and
- (d) identifying modified anthocyanins with improved stability, color, or hue.
A method of identifying modified anthocyanins with improved stability, color, or hue using a screening method comprising the steps of:
- (a) providing one or more microbial host cells capable of producing at least one anthocyanidin, wherein said host cells comprise one or more heterologous glycosyltransferase (GT) nucleic acid molecules, one or more heterologous acyltransferase (AT) nucleic acid molecules, or both one or more heterologous GT nucleic acid molecules and one or more heterologous AT nucleic acid molecules;
- (b) cultivating said host cells under conditions allowing growth of said cells and production of at least one anthocyanin thereby;
- (c) screening said host cells and/or culture medium for modified anthocyanins with improved stability, color, or hue;
- wherein the screening comprises:
- (i) isolating one or more anthocyanins from said host cells and/or from culture medium of the host cells;
- (ii) exposing the one or more anthocyanins to steadily increasing concentrations of a reagent to effect a change in the color intensity of the one or more anthocyanins;
- (iii) comparing the concentration where a loss of color intensity occurs with a control, wherein the ability to retain color intensity at higher concentrations of exposure to the reagent than the control indicates improved anthocyanin stability
- and
- (d) identifying modified anthocyanins with improved stability, color, or hue.
A method of identifying a host cell capable of producing a modified anthocyanin with a predetermined property, said method comprising the steps of:
- (a) providing a composition of microbial host cells, wherein each host cell is capable of producing at least one anthocyanidin, and wherein each host cell comprises one or more heterologous glycosyltransferase (GT) nucleic acid molecules and one or more heterologous acyltransferase (AT) AT nucleic acid molecules;
- (b) cultivating said host cells under conditions allowing growth of said host cells and production of at least one anthocyanin thereby;
- (c) screening the composition of host cells, for host cells producing a modified anthocyanin with the predetermined property;
- and
- (d) selecting said host cell;
- wherein a host cell capable of producing a modified anthocyanin with a predetermined property is identified.
A method of producing modified anthocyanins with improved stability, color, or hue, said method comprising the steps of:
- (a) providing two or more microbial host cells capable of producing at least one anthocyanidin, wherein each host cell comprises one or more heterologous GT nucleic acid molecules and one or more heterologous AT nucleic acid molecules;
- (b) cultivating said host cells under conditions allowing growth of said cells and production of modified anthocyanins thereby;
- and
- (c) isolating one or more modified anthocyanins from the host cells and/or from the culture medium.
Dependencies are inferred from the claim text.