Engineered organisms for production of novel lipids

An engineered microorganism comprising a multi-methyl branched fatty acid (MMBFA) ester synthesis pathway comprising:

• (i) an exogenous or overexpressed mycocerosic acid synthase (MAS) or active fragment thereof comprising an ACP domain;
• (ii) an exogenous or overexpressed long chain fatty acid AMP ligase;
• and
• (iii) an exogenous or overexpressed ester synthase,
• wherein the MAS catalyzes the iterative conversion of methyl-malonyl-CoA to MMBFA, and the ester synthase catalyzes the transesterification of MMBFA covalently bound to the ACP domain of MAS with an acceptor alcohol to produce an MMBFA ester,
• wherein said engineered microorganism further comprises:
• (iv) an exogenous or overexpressed propionyl-CoA carboxylase (PCC) complex comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO:
• 81, an amino acid sequence having at least 80% sequence identity to SEQ ID NO:
• 83, and an amino acid sequence having at least 80% sequence identity to SEQ ID NO:
• 84;
• and/or
• (v) an exogenous or overexpressed acyl-acyl carrier protein (ACP) thioesterase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO:
• 89.