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US 10,119,129 B2Granted
Enzyme complex from Trichoderma reesei and P. funiculosum enzymes
AssigneeDupont Nutrition Biosciences Aps
InventorsNeville Marshall Fish, Lone Brønd Miller
Life of this patent
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Abstract
The invention relates to an improved enzyme complex having a plurality of enzyme activities of an expression product obtained by fermentation of the genus Trichoderma in combination with one or more enzymes of a different fungus strain.
Claims14
An enzyme complex having beta-1,4-endoglucan hydrolase activity comprising:
- a. a fermentation product of Trichoderma reesei wherein at least 61 % of the beta-1,4-endoglucan hydrolase activity of the enzyme complex, as measured by the “Assay 1” method, is from the fermentation product of Trichoderma reesei;
- and
- b. one or more enzymes derived from Penicillium funiculosum selected from a xylanase (EC 3.2.1.8), a cellulase (EC 3.2.1.4), and a beta-glucanase (EC 3.2.1.6), wherein 30% to 39% of the beta-1,4-endoglucan hydrolase activity of the enzyme complex, as measured by the “Assay 1” method, is from a fermentation product of Penicillium funiculosum;
- wherein the enzyme complex is substantially cell free.
1 more element
An enzyme complex having beta-1,4-endoglucan hydrolase activity comprising:
- a. a first expression product obtained by fermentation of Trichoderma reesei, wherein the first expression product accounts for at least 61% of the beta-1,4-endoglucan hydrolase activity of the enzyme complex;
- and
- b. a second expression product obtained by fermentation of Penicillium funiculosum, wherein the second expression product accounts for at least 39% of the beta-1,4-endoglucan hydrolase activity of the enzyme complex;
- wherein the percentages of beta-1,4-endoglucan hydrolase activity are measured by the “Assay 1” method;
- wherein the enzyme complex is substantially cell free.
2 more elements
Dependencies are inferred from the claim text.