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US 10,119,128 B2Granted
Lipase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsLuise Erlandsen, Carsten Hoerslev Hansen, Jesper Vind, Allan Svendsen, Carsten Peter Sonksen
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Abstract
The present invention relates to lipase variants and methods of obtaining them. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims7
A lipase variant, comprising a substitution at a position corresponding to position G91A of the mature polypeptide of SEQ ID NO:
- 2, wherein the variant has at least 90% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, has lipase activity and, in comparison with the parent lipase, has improved performance in the presence of an organic catalyst selected from the group consisting of organic catalysts having the formulae (a)-(c):
- and
- c) mixtures thereof;
- wherein each R1 is independently a branched alkyl group containing from 3 to 24 carbons or a linear alkyl group containing from 1 to 24 carbons;
- and
- wherein said lipase variant is selected from the group consisting of the substitutions (d)-(g):
- d) T37R+N39R+G91A+D96G+T231R+N233R;
- e) G91A+D96G+T231R+N233R;
- f) G91A+D96G+G225R+T231R+N233R;
- and
- g) G91A+D96G+A150G+T231R+N233R.
9 more elements
Dependencies are inferred from the claim text.