Enzymes, enzyme components and uses thereof
AssigneeBASF Plant Science GmbH
InventorsLaurent Marty, Toralf Senger
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Abstract
The invention provides nucleic acid molecules which encodes a novel fatty acid desaturase, KCS, KCR and/or LACS from Thraustochytrium aureum and Sphaeroforma arctica. The invention also provides recombinant expression vectors containing the nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA and for screening for delta-4 desaturases.
Claims8
A method for increasing the efficiency of eicosapentaenoic acid (EPA) production in a host cell capable of synthesizing EPA, the method comprising expressing in the cell a heterologous lysophospholipid-coenzyme A synthase (LACS) enzyme having at least 80% sequence identity to SEQ ID NO:
- 84 and fatty acyl CoA synthetase activity.
A plant comprising a host cell capable of synthesizing EPA, the host cell comprising a polynucleotide encoding a heterologous LACS enzyme having at least 80% sequence identity to SEQ ID NO:
- 84 and fatty acyl CoA synthetase activity and further comprising one or more polynucleotides encoding a delta-5 elongase, a delta-6 elongase, a delta-5 desaturase, and/or a delta-6 desaturase.
A seed comprising a host cell capable of synthesizing EPA, the host cell comprising a polynucleotide encoding a heterologous LACS enzyme having at least 80% sequence identity to SEQ ID NO:
- 84 and fatty acyl CoA synthetase activity and further comprising one or more polynucleotides encoding a delta-5 elongase, a delta-6 elongase, a delta-5 desaturase, and/or a delta-6 desaturase.
A method for increasing the efficiency of docosahexaenoic acid (DHA) production in a host cell capable of synthesizing DHA, the method comprising expressing in the cell a heterologous lysophospholipid-coenzyme A synthase (LACS) enzyme having at least 80% sequence identity to SEQ ID NO:
- 84 and fatty acyl CoA synthetase activity.
A plant comprising a host cell capable of synthesizing DHA, the host cell comprising a polynucleotide encoding a heterologous LACS enzyme having at least 80% sequence identity to SEQ ID NO:
- 84 and fatty acyl CoA synthetase activity and further comprising one or more polynucleotides encoding a delta-5 elongase, a delta-6 elongase, a delta-5 desaturase, and/or a delta-6 desaturase.
A seed comprising a host cell capable of synthesizing DHA, the host cell comprising a polynucleotide encoding a heterologous LACS enzyme having at least 80% sequence identity to SEQ ID NO:
- 84 and fatty acyl CoA synthetase activity and further comprising one or more polynucleotides encoding a delta-5 elongase, a delta-6 elongase, a delta-5 desaturase, and/or a delta-6 desaturase.
Dependencies are inferred from the claim text.