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US 10,119,121 B2Granted
Ex vivo proliferation of epithelial cells
AssigneePropagenix Inc.
InventorChengkang Zhang
Life of this patent
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Abstract
The technology relates in part to methods and compositions for ex vivo proliferation and expansion of epithelial cells.
Claims44
A method for proliferating epithelial cells ex vivo, comprising:
- expanding the number of cells in an originating cell population comprising one or more of differentiated epithelial cells, transit-amplifying epithelial cells, lineage-committed epithelial cells, and basal epithelial cells under expansion culture conditions, thereby generating an expanded epithelial cell population, wherein:
- the expansion culture conditions comprise a transforming growth factor beta (TGF-beta) inhibitor and an agent that disrupts cytoskeletal structure;
- and
- the expansion culture conditions are serum-free and feeder-cell free culture conditions.
1 more element
A method for proliferating epithelial cells ex vivo, comprising:
- expanding the number of cells in an originating cell population comprising one or more of differentiated epithelial cells, transit-amplifying epithelial cells, lineage-committed epithelial cells, and basal epithelial cells under expansion culture conditions, thereby generating an expanded epithelial cell population, wherein:
- the expansion culture conditions comprise components consisting essentially of i) a transforming growth factor beta (TGF-beta) signaling inhibitor;
- ii) a Rho-associated protein kinase inhibitor, a p21-activated kinase (PAK) inhibitor, or a myosin II inhibitor;
- iii) a mitogenic growth factor;
- iv) a beta-adrenergic receptor agonist;
- v) calcium at a concentration below 100 μM;
- and vi) a serum-free base medium;
- and
- the expansion culture conditions are serum-free and feeder-cell free culture conditions.
6 more elements
A population of ex vivo expanded epithelial cells produced by a method comprising:
- expanding the number of cells in an originating cell population comprising one or more of differentiated epithelial cells, transit-amplifying epithelial cells, lineage-committed epithelial cells, and basal epithelial cells under expansion culture conditions, thereby generating an expanded epithelial cell population, wherein:
- the expansion culture conditions comprise a transforming growth factor beta (TGF-beta) inhibitor and an agent that disrupts cytoskeletal structure;
- and
- the expansion culture conditions are serum-free and feeder-cell free culture conditions.
1 more element
A method for genetically modifying cells, comprising contacting cells with one or more agents that introduce a genetic modification to the cells, wherein the cells have been expanded by a method comprising:
- expanding the number of cells in an originating cell population comprising one or more of differentiated epithelial cells, transit-amplifying epithelial cells, lineage-committed epithelial cells, and basal epithelial cells under expansion culture conditions, thereby generating an expanded epithelial cell population, wherein:
- the expansion culture conditions comprise a transforming growth factor beta (TGF-beta) inhibitor and an agent that disrupts cytoskeletal structure;
- and
- the expansion culture conditions are serum-free and feeder-cell free culture conditions.
1 more element
Dependencies are inferred from the claim text.