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US 10,119,120 B2Granted
Method for inducing differentiation of pluripotent stem cells into neural precursor cells
AssigneeKyoto University
InventorsJun Takahashi, Asuka Morizane
Life of this patent
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Abstract
The present invention provides a method for inducing differentiation of pluripotent stem cells into neural precursor cells, comprising culturing the pluripotent stem cells in the presence of a small molecule BMP inhibitor, and induced neural precursor cells prepared by this method.
Claims10
A method for inducing differentiation of a pluripotent cell into a neural precursor cell, the method comprising:
- treating the pluripotent stem cell with a p160-Rho-associated coiled-coil kinase (ROCK) inhibitor;
- and
- culturing the treated pluripotent stem cell for inducing the differentiation into the neural precursor cell in a culture medium comprising substances consisting of 4-(6-(4-(piperazin-1-yl)phenyl)pyrazolo[1,5-a]pyrimidin-3-yl)quinoline (LDN-193189) and a TGFβ family inhibitor selected from the group consisting of 4-[4-(1,3-benzodioxol-5-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]benzamide (SB431542) and 3-(6-methylpyridin-2-yl)-1-phenylthiocarbamoyl-4-quinolin-4-ylpyrazole (A-83-01), wherein the concentration of LDN-193189 is 50 nM or higher.
Dependencies are inferred from the claim text.