Methods and compositions for producing solvents
AssigneesThe Board of Trustees of the University of Illinois, Eastman Renewable Materials, LLC
InventorsHans P. Blaschek, Steven F. Stoddard, Zhen Shi
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Abstract
Described herein are methods, compositions and synthetic biology approaches for solvent production, including but not limited to butanol production. Described herein are recombinant bacteria and yeast strains which may be used in production of a solvent, including but not limited to butanol, from lignocellulosic and other plant-based feedstocks. Described herein are methods of producing solvents, including but not limited to butanol, using bacteria and yeast strains. Described herein are methods of producing organisms that display highly efficient butanol production.
Claims16
A recombinant solventogenic microorganism, wherein the microorganism is transformed with:
- (a) a nucleic acid molecule comprising at least one Clostridium beijerinckii Adh (alcohol dehydrogenase) polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO:
- 14, wherein the polynucleotide encodes a polypeptide having alcohol dehydrogenase activity;
- (b) a nucleic acid molecule comprising at least one Clostridium beijerinckii Bcd (butyryl-CoA dehydrogenase) polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO:
- 2, wherein the polynucleotide encodes a polypeptide having butyryl-CoA dehydrogenase activity;
- and
- (c) a nucleic acid molecule comprising at least one Clostridium beijerinckii Buk (butyrate kinase) polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO:
- 3, wherein the polynucleotide encodes a polypeptide having butyrate kinase activity;
- wherein the recombinant solventogenic microorganism is capable of (i) more efficient butanol production, (ii) faster butanol production, and/or (iii) increased butanol production relative to the same recombinant solventogenic microorganism that is not transformed with the nucleic acid molecules of (a), (b), and (c).
Dependencies are inferred from the claim text.