Device for the lysis of microorganisms present in an environmental or clinical sample and the extraction of nucleic acids from said microorganisms for analysis
AssigneeBiomerieux
InventorsHermanus Johannes Maria Kreuwel, Emiel Gerebern Maria Verwimp
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Abstract
A cartridge that can be positioned inside an air collection component and receive a component for recovering nucleic acids is described, the cartridge being substantially cylindrical and containing a microorganism retaining zone, the retaining zone containing a microorganism lysis mechanism. Also described is a device for collecting microorganisms contained in the air and a device for microorganism lysis.
Claims34
A cartridge which can be positioned inside an air collection device and receive a means for recovering nucleic acids, said cartridge being substantially cylindrical and having an upper end and lower end, said cartridge comprising a microorganism retaining zone, said retaining zone comprising microorganism lysis means for lysing the microorganisms, wherein
- the upper end of the cartridge is open,
- the lower end is constituted of a wall, having, in its center, an orifice, there being, along an extension of said orifice, a duct extending, inside said cartridge, and
- the microorganism retaining zone comprises a material configured to retain the microorganisms, configured to keep the lysis means in place, and configured to dissolve in the presence of a liquid to release the lysis means from the material.
Dependencies are inferred from the claim text.