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US 10,119,029 B2Granted
Method of isolating blue anthocyanin fractions
AssigneesMars, Incorporated, The Ohio State University
InventorsRebecca J. Robbins, J. Christopher Johnson, Thomas M. Collins, Neda Ahmadiani, M. Monica Giusti
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Abstract
The present invention is directed to a method of isolating fractions of anthocyanin molecules from anthocyanin-containing vegetable and fruit juices and extracts, or combinations thereof, at a select pH based on differences in polarity of the anthocyanin molecules in the anthocyanin-containing vegetable and fruit juices and extracts.
Claims16
A method of isolating a fraction of anthocyanins from an anthocyanin-containing vegetable or fruit juice or extract, or a combination thereof, comprising:
- a) loading an anthocyanin-containing vegetable or fruit juice or extract, or a combination thereof, on an ion exchange column;
- b) selectively separating anthocyanins into two or more fractions on the ion exchange column based on differences in charge and polarity of the anthocyanin molecules using a solvent of select pH, wherein the pH is from 2 to less than 4;
- c) selecting one fraction or a combination of fractions containing separated anthocyanins, such that the separated anthocyanins in the one fraction or the combination of fractions, when in an aqueous solution at pH 8.0 has a maximum absorbance of 615 nm to 635 nm;
- and
- d) adjusting a pH of the one fraction or the combination of fractions to obtain a solution comprising separated anthocyanins having a maximum absorbance of 615 nm to 635 nm.
2 more elements
Dependencies are inferred from the claim text.