Multimerization of recombinant protein by fusion to a sequence from lamprey
AssigneeSanofi Pasteur Limited
InventorsRégis Sodoyer, Isabelle Legastelois
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Abstract
The present invention relates to polymerized recombinant proteins, to recombinant nucleic acids coding for the polymerized recombinant proteins, to expression cassettes comprising the recombinant nucleic acids, to host cells transformed by the expression cassettes and to a method for multimerizing a recombinant protein. The polymerized proteins of the invention may be used in pharmaceutical or immunogenic compositions. In particular, the recombinant proteins may be antigens, antibodies or scaffolds. In particular, the polymerized recombinant protein may be an influenza haemagglutinin.
Claims22
A molecule which comprises a first amino acid sequence which has at least 80% identity to SEQ ID NO:
- 1 and a second amino acid sequence which is heterologous to said first sequence, wherein said molecule does not comprise a leucine-rich repeat (LRR) module from a lamprey VLR-B antibody.
A recombinant nucleic acid which comprises a first nucleic acid sequence with at least 80% identity to SEQ ID NO:
- 3 and a second nucleic acid sequence which is heterologous to said first sequence, wherein said recombinant nucleic acid does not encode a leucine-rich repeat (LRR) module from a lamprey VLR-B antibody.
A method for multimerizing a recombinant protein comprising:
- a) fusing a nucleic acid sequence having at least 80% identity to SEQ ID NO:
- 3 to the nucleic acid sequence coding for said recombinant protein, with the proviso that said recombinant protein does not comprise a leucine-rich repeat (LRR) module from a lamprey VLR-B antibody,
- b) expressing the fusion protein encoded by said nucleic acid sequence, under conditions which lead to the multimerization of said recombinant protein.
Dependencies are inferred from the claim text.