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AssigneeNorthwestern University
InventorsMichael Christoper Jewett, Rey William Martin, Seok Hoon Hong, Yong Chan Kwon, Benjamin James Des Soye
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The invention relates to genomically recoded organisms, platforms for preparing sequence defined biopolymers in vitro comprising a cellular extract from a genomically recorded organism, and methods for preparing sequence defined biopolymers in vitro are described. In particular, the invention relates to genomically recoded organisms comprising a strain deficient in release factor 1 (RF-1) or a genetic homolog thereof and at least one of at least one additional genetic knock-out mutation, at least one additional upregulated gene product, or both at least one additional knock-out mutation and at least one additional upregulated gene product.
A platform for preparing a sequence defined biopolymer or a protein in vitro, the platform comprising a cellular extract from a genomically recoded E. coli strain comprising:
A platform for preparing a sequence defined biopolymer or a protein in vitro, the platform comprising a cellular extract from a genomically recoded E. colistrain comprising a genetic knock-out mutation in release factor 1 (RF-1) or a genetic homolog thereof and the genomically recoded strain further comprising at least one additional upregulated gene product, wherein the at least one addition upregulated gene product improves energy supply, chaperone levels, translation function, ribosome recycling, or any combination thereof, and the at least one additional upregulated gene product comprises a member selected from the group consisting of ackA, ndk, pykF, cdd, dsbC, dnaK, dnaJ, crpE, tig, groS, groL, infA, infB, fusA, efp, lepA, tufB, hslR, ffr, and any combination thereof.
A platform for preparing a sequence defined biopolymer or a protein in vitro, the platform comprising a cellular extract from a genomically recoded E. colistrain comprising:
A platform for preparing a sequence defined biopolymer or a protein in vitro, the platform comprising a cellular extract from a genomically recoded E. colistrain comprising:
Dependencies are inferred from the claim text.