Compositions and methods to treat herpes simplex virus infections
AssigneeAgenovir Corporation
InventorsStephen R. Quake, Jianbin Wang
Life of this patent
Estimating the term…
Abstract
Herpes simplex virus (HSV) including herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are a persistent cause of human disease with no known cure. Guided nuclease systems target specific regions of the HSV-1 and HSV-2 genomes, disrupting the virus' nucleic acid and rendering even latent viruses incapacitated.
Claims21
A composition for treatment of a herpes simplex virus (HSV) infection, the composition comprising a vector encoding:
- a Cas9 endonuclease;
- and
- a guide RNA having a portion complementary to an origin of replication S (oriS) region of HSV nucleic acid, the guide RNA capable of directing the Cas9 endonuclease to the region of HSV nucleic acid.
A method for treating a herpes simplex virus (HSV) infection, the method comprising:
- introducing into a cell of a host,
- (i) a vector encoding a Cas9 endonuclease and a guide RNA having a portion complementary to an origin of replication S (oriS) region of HSV nucleic acid, or
- (ii) a ribonucleoprotein that includes the Cas9 endonuclease and a guide RNA having a portion complementary to an origin of replication S (oriS) region of HSV nucleic acid, wherein the guide RNA is capable of directing the Cas9 endonuclease to the region of HSV nucleic acid.
A composition for treatment of a herpes simplex virus (HSV) infection, the composition comprising a ribonucleoprotein, wherein the ribonucleoprotein comprises:
- a Cas9 endonuclease;
- and
- a guide RNA having a portion complementary to an origin of replication S (oriS) region of HSV nucleic acid, the guide RNA capable of directing the Cas9 endonuclease to the region of HSV nucleic acid.
A composition for treatment of a herpes simplex virus (HSV) infection, the composition comprising:
- an mRNA encoding a Cas9 endonuclease;
- and
- a guide RNA having a portion complementary to an origin of replication S (oriS) region of HSV nucleic acid, the guide RNA capable of directing the Cas9endonuclease to the region of HSV nucleic acid.
Dependencies are inferred from the claim text.