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US 10,117,452 B2Granted
Glucosyl stevia composition
AssigneePureCircle Sdn Bhd
InventorAvetik Markosyan
Life of this patent
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Abstract
Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.
Claims12
A process for producing a highly purified glucosyl stevia composition, comprising the steps of:
- adding starch into water to form a starch suspension;
- adding a mixture of α-amylase and CGTase into the starch suspension and incubating for about 0.5 to 2 hours at about 75-80° C., resulting in a liquefied starch suspension;
- inactivating the α-amylase by low pH heat treatment;
- cooling the liquefied starch suspension and adjusting the pH to about 5.5 to 7.0;
- adding steviol glycosides into the liquefied starch suspension, resulting in a reaction mixture;
- adding a second batch of CGTase into the reaction mixture and incubating for about 12 to 48 hours at about 55-75° C.;
- adding a second batch of α-amylase into the reaction mixture and incubating for a further about 12-16 hours at about 55-95° C.;
- inactivating the second batch of α-amylase in the reaction mixture by low pH heat treatment;
- and
- removing impurities from the reaction mixture by contacting the reaction mixture with macroporous adsorbent resin and subsequently eluting adsorbed diterpene glycosides with aqueous ethanol to result in a purified reaction mixture;
- wherein the purified reaction mixture comprises α-1,4-glucosyl derivatives, and unmodified steviol glycosides.
8 more elements
Dependencies are inferred from the claim text.