Method of collecting placental stem cells
AssigneeCelularity Inc.
InventorRobert J. Hariri
Life of this patent
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Abstract
A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood by perfusing the drained placenta with an anticoagulant solution to flush out residual cells, collecting the residual cells and perfusion liquid from the drained placenta, and separating the embryonic-like cells from the residual cells and perfusion liquid. Exogenous cells can be propagated in the placental bioreactor and bioactive molecules collected therefrom.
Claims24
A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood said method comprising:
- perfusing a placenta which has been drained of cord blood with an anticoagulant perfusion solution to flush out residual cells and embryonic-like stem cells from said drained placenta, and
- collecting said residual cells and embryonic-like stem cells and perfusion solution from the drained placenta.
A method of propagating exogenous cells in a placental bioreactor which has been treated to remove residual umbilical cord blood, comprising:
- perfusing a placenta which has been drained of cord blood with an anticoagulant perfusion solution to flush out residual cells and embryonic-like stem cells,
- collecting said residual cells, said embryonic-like stem cells, and said anticoagulant perfusion solution from the drained placenta,
- removing all remaining viable endogenous cells from the drained placenta,
- perfusing the placenta with nutrient perfusion solution,
- introducing exogenous cells into the perfused placenta,
- incubating the perfused placenta with exogenous cells, and
- collecting propagated exogenous cells from the perfused placenta.
Dependencies are inferred from the claim text.