Method for culturing microalgae of the aurantiochytrium genus in a culture medium without chloride and without sodium for the production of DHA

A method for producing DHA in a culture of protists of the genus Aurantiochytrium comprising the following step:

• a) culturing, in heterotrophic or mixotrophic conditions, one or more strains of the genus Aurantiochytrium in a chemically defined culture medium,
• b) maintaining said culture for several generations, and
• c) recovering the biomass thus cultured,
• wherein
• the one or more strains of the genus Aurantiochytrium has a genetic identity of at least 92% to sequence SEQ ID NO:
• 1 and
• the chemically defined culture medium has less than 3.5 g/L of sodium ions and less than 1 g/L of chloride ions and has 200 mM to 500 mM organic carbon-containing substrate.