Methods and compositions for protein delivery
AssigneeThe United States of America, as represented by the Secretary, Dept. of Health and Human Services
InventorsDeb Chatterjee, Stanislaw Jan Kaczmarczyk
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Abstract
The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
Claims20
A VLP comprising:
- a Gag fusion protein comprising a matrix protein, a capsid protein, a nucleocapsid protein, covalently linked to a protein of interest selected from the group consisting of a cytotoxic enzyme, an interferon, a tumor suppressor, a recombinase, a hormone, and a stem cell transcription factor;
- a fusogenic protein having reduced ligand-binding activity;
- and
- a nuclear localization signal (NLS) comprising at least a portion of SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
A method of targeting one or more proteins to a cell, comprising contacting a cell with a VLP comprising:
- a Gag fusion protein comprising a matrix protein, a capsid protein, a nucleocapsid protein, covalently linked to a protein of interest selected from the group consisting of a cytotoxic enzyme, an interferon, a tumor suppressor, a recombinase, a hormone, and a stem cell transcription factor;
- a fusogenic protein having reduced ligand-binding activity;
- and,
- a nuclear localization signal comprising at least a portion of SEQ ID NO:
- 1 or SEQ ID NO:
- 2,
- thereby targeting one or more proteins to the cell.
A method of providing protein therapy to a target cell, comprising contacting the cell with a VLP comprising:
- a Gag fusion protein comprising a matrix protein, a capsid protein, a nucleocapsid protein, covalently linked to a protein of interest selected from the group consisting of a cytotoxic enzyme, an interferon, a tumor suppressor, a recombinase, a hormone, and a stem cell transcription factor;
- a fusogenic protein having reduced ligand-binding activity;
- and,
- a nuclear localization signal comprising at least a portion of SEQ ID NO:
- 1 or SEQ ID NO:
- 2,
- thereby providing protein therapy to the target cell.
Dependencies are inferred from the claim text.