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AssigneesUniversity of Miami, President and Fellows of Harvard College
InventorsRonald Desrosiers, Young Shin
Estimating the term…
Compositions are provided comprising a replication-competent herpesvirus vector system and/or a herpesvirus vector particle comprising a heterologous recombinant transgene of interest encoding an antigen of interest (e.g., a tumor-associated antigen, a microbial antigen, etc.), wherein the recombinant transgene has been modified to comprise a codon usage signature of a herpesvirus late gene operably linked to an active promoter. Such compositions find use in a variety of methods including, for example, methods of generating an immune response against an antigen of interest in a subject in need thereof, as well as methods for treating or preventing cancer or a microbial infection.
A replication-competent herpesvirus vector system comprising (a) a polynucleotide encoding a trans-inducer polypeptide that enhances expression of a herpesvirus late gene, and (b) a polynucleotide comprising a heterologous recombinant transgene of interest operably linked to a promoter, wherein said heterologous recombinant transgene of interest comprises a codon usage signature of the herpesvirus late gene.
A replication-competent herpesvirus vector particle comprising within its genome a polynucleotide comprising a sequence encoding a trans-inducer polypeptide that enhances expression of a herpesvirus late gene and a heterologous recombinant transgene of interest operably linked to a promoter, wherein said heterologous recombinant transgene comprises a codon usage signature of the herpesvirus late gene.
A method of increasing expression of a transgene by a host cell, comprising (a) altering codons of the transgene to reflect the codon usage signature of a herpesvirus late gene, (b) inserting the transgene with the altered codons into a replication-competent herpesvirus vector, (c) introducing into the host cell the replication-competent herpesvirus vector, and (d) culturing the host cell comprising the replication-competent herpesvirus vector in the presence of a herpesvirus trans-inducer polypeptide that enhances expression of the herpesvirus late gene.
Dependencies are inferred from the claim text.