Abstract
Dissociations of multiple-charged ions are detected and analyzed by charge-separation tandem mass spectrometry. Analyte molecules are ionized to form multiple-charged parent ions. A particular charge parent ion state is selected in a first-stage mass spectrometer and its mass-to-charge ratio (M/Z) is detected to determine its mass and charge. The selected parent ions are then dissociated, each into a plurality of fragments including a set of daughter ions each having a mass of at least one molecular weight and a charge of at least one. Sets of daughter ions resulting from the dissociation of one parent ion (sibling ions) vary in number but typically include two to four ions, one or more multiply-charged. A second stage mass spectrometer detects mass-to-charge ratio (m/z) of the daughter ions and a temporal or temporo-spatial relationship among them. This relationship is used to correlate the daughter ions to determine which (m/z) ratios belong to a set of sibling ions. Values of mass and charge of each of the sibling ions are determined simultaneously from their respective (m/z) ratios such that the sibling ion charges are integers and sum to the parent ion charge.
Key facts
- Application
- 07/530,667Filed 29 May 1990
- Granted
- 17 Dec 1991
- Term ends
- Not estimatedThe source records don’t support an estimate.
Classification4 codes
- H01J 49/40
Dynamic spectrometers: time-of-flight spectrometers
- H01J 49/004
Particle spectrometers or separator tubes: combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
- H01J 49/32
Static spectrometers: using double focusing
- H01J
Electric discharge tubes or discharge lamps
Family
No related US applications are recorded in the indexed filing history.