Back to results
US RE51,046 E1Granted
Polypeptide variants
AssigneeNovozymes A/S
InventorsLars Beier, Lars Henrik Oestergaard, Annette Helle Johansen, Klaus Gori, Thomas Holberg Blicher, Gernot Abel, Steen Krogsgaard, Jens E. Nielsen, Lars Giger
Life of this patent
Estimating the term…
Abstract
The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims31
A variant of a DNase parent, wherein the variant comprises one or both motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO:
- 25) or ASXNRSKG (SEQ ID NO:
- 26),
- wherein the variant comprises one or more substitution(s) compared to SEQ ID NO:
- 1, wherein the substitution is one or more substitution(s) are selected from the group consisting of:
- T1I, T1V, T1Y, T1M, or T1E,
- G4N,
- T5F, T5F or T5C,
- P6V, P6V or P6G,
- S7D, S7T,
- K8V,
- S9K, S9Q, S9V, S9L, S9F, S9P, or S9R,
- A10D, A10M, A10I, A10Q, A10V, A10L, or A10K,
- Q12S, Q12V, or Q12E,
- Q14M, Q14M or Q14R,
- N16S,
- A17C, A17V, A17E, or A17T,
- T19K, T19L, or T19S, T19I, T19V,
- K21E, K21E or K21M,
- G24Y,
- S25P,
- S27N, S27I, S27M, S27D, S27V, S27F, S27A, S27C, S27L, or S27E,
- G28L,
- Y29W,
- S30K, S30D, S30H, or S30T,
- D32Q,
- I38V, I38V or I38M,
- Q40V,
- S42C, S42L, S42M, S42F, or S42W,
- V49R,
- L51I,
- K52I, K51I or K52H,
- A55S,
- D56I, D56L, or D56T,
- S57W, S57F, S57H, S57C, S57P, S57V, S57R, or S57T,
- Y58A, Y58A or Y58T,
- S59C, S59T, S59L, S59Q, S59V, S59K, S59R, S59M, S59I, or S59H,
- N61D,
- P63A,
- T65L, T65I, T65V, T65R, or T65K,
- S68V, S68I, S68W, S68Y, S68H, S68C, S68T, or S68L,
- V76G, V76L, V76C, V76K, V76H, V76E, V76A, V76Y, V76N, V76M, V76R, V76F, or
- T77N, T77Y, T77W, or T77R,
- F78I, F78H, F78Y, F78C,
- T79G, T79G or T79R,
- N80K,
- S82L, S82E, S82K, S82R, or S82H,
- D83C, D83F, or D83L,
- L92T,
- A93G,
- E94N,
- G99S,
- S101D, S101D or S101A,
- S102M, S102L, S102V, S102A, S102K, S102T, or S102R,
- T104P, T104P or T104A,
- T105V, T105V or T105I,
- K107L, K107C, K107R, K107H, K107S, K107M, K107E, K107A, or K107D,
- Q109R, Q109S,
- A112S,
- S116D, S116R, S116Q, S116H, S116V, S116A, S116E, or S116K,
- A125K,
- S126I, S126E, S126A, or S126C,
- T127C, T127V,
- S130E,
- G132R,
- D135R,
- T138Q,
- W139R,
- R143E, R143E or R143K,
- S144Q, S144H, S144L, S144P, S144E, or S144K,
- G145V, G145E, or G145D, G145A,
- A147Q, A147Q or A147W, A147S,
- G149S,
- K152H, K152H or K152R,
- S156C, S156G, S156K, S156R, S156T, or S156A,
- T157S,
- Y159F,
- K160V,
- W161L, W161L or W161Y,
- G162Q, G162D, G162M, G162R, G162A, G162S, G162E, G162L, G162K, G162V, or G162H,
- S164R, S164R or S164T,
- Q166D,
- S167M, S167L, S167F, S167W, S167E, S167A, S167Y, S167H, S167C, S1671, S167Q, S167V, or S167T,
- S168V, S168E, S168D, or S168L,
- K170S, K170L, K170F, or K170R,
- T171D, T171E, T171A, or T171C,
- A172G, A172S,
- L173T, L173A, or L173V,
- Q174L,
- G175D, G175E, G175N, or G175R,
- M176H,
- L177I,
- N178D, N178E, N178T, N178S, or N178A,
- S179E,
- S181R, S181E, S181D, S181F, S181H, S181W, S181L, S181M, S181Y, S181Q, S181G, or S181A, and
- Y182M, Y182C, Y182K, Y182G, Y182A, Y182S, Y182V, Y182D, Y182Q, Y182F, Y182L, Y182N, Y1821, Y182E, Y182T and or Y182W,
- wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO:
- 1 of at least 80% and the variant has DNase activity.
94 more elements
A method for obtaining a DNase variant, comprising:
- a) introducing into a parent DNase an alteration at one or more positions corresponding to positions 1, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 17, 19, 21, 24, 25, 27, 28, 29, 30, 32, 38, 40, 42, 49, 51, 52, 55, 56, 57, 58, 59, 61, 63, 65, 68, 76, 77, 78, 79, 80, 82, 83, 92, 93, 94, 99, 101, 102, 104, 105, 107, 109, 112, 116, 125, 126, 127, 130, 132, 135, 138, 139, 143, 144, 145, 147, 149, 152, 156, 157, 159, 160, 161, 162, 164, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 181 and 182 of SEQ ID NO 1, wherein the variant has DNase activity;
- and
- b) recovering the variant.
A variant of a DNase parent, wherein the variant comprises one or both motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO:
- 25) or ASXNRSKG (SEQ ID NO:
- 26),
- wherein the variant comprises one or more substitution(s) compared to SEQ ID NO:
- 1, wherein the one or more substitution(s) are selected from the group consisting of:
- S7T,
- T19I or T19V,
- S27I,
- S59V,
- V76L,
- F78I,
- K107R,
- Q109R,
- T127V,
- A147S,
- G162D, and
- A172S,
- wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO:
- 1 of at least 85% and the variant has DNase activity.
15 more elements
Dependencies are inferred from the claim text.