Systems and methods for tissue processing and preparation of cell suspension therefrom
AssigneeAVITA Medical Americas, LLC
InventorsAndrew Perry Quick, David Allen Fencil, William Ford Dolphin
Life of this patent
Estimating the term…
Abstract
The present invention provides for methods and at least partially automated devices suitable for producing a transplantable cellular suspension of living tissue suitable for promoting tissue regeneration in an epithelium-related procedure, as well as compositions produced therefrom. Tissue regeneration in humans is extremely limited and constitutes a major challenge to the repair of damaged organ function. Wound treatment is a typical area where tissue regeneration is required. Wounds (lacerations or openings) in mammalian tissue can result in tissue disruption and coagulation of the microvasculature at the wound face.
Claims36
A device for preparing cells for transplant, the device comprising:
- a tissue processing chamber configured to receive a tissue and a chemical reagent, wherein the chemical reagent chemically dissociates the tissue;
- a reciprocating-rotating pestle in the tissue processing chamber, wherein the reciprocating-rotating pestle is actuatable to exert a grinding force on the tissue, wherein the force mechanically dissociates the tissue;
- and
- a cell collection chamber configured to collect a cell suspension comprising the chemically and mechanically dissociated tissue from the tissue processing chamber.
A method for preparing cells for transplant, the method comprising:
- placing a tissue in a tissue processing chamber of a cartridge, the tissue processing chamber comprising a disintegrator on which the tissue sits, the tissue processing chamber configured to receive a chemical reagent, wherein the chemical reagent is configured to chemically dissociate the tissue;
- sealing the tissue processing chamber;
- placing the cartridge within a console for removably housing the cartridge;
- directing a processor in the console to actuate a disintegrating member such that compression and rotational force is exerted on the tissue within the tissue processing chamber containing the tissue and the chemical reagent to chemically and mechanically dissociate the tissue, wherein the disintegrating member does not contact the tissue;
- collecting a cell suspension in a cell collection chamber of the cartridge;
- and
- retrieving a disposable applicator having the cell suspension therein.
Dependencies are inferred from the claim text.