Musculoskeletal stem cell and medium for inducing differentiation of musculoskeletal stem cell
AssigneeCellatoz Therapeutics, Inc.
InventorMyung-Kwan Han
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Abstract
The present disclosure relates to a novel musculoskeletal stem cell (MSSC) differentiated from an ESC (embryonic stem cell) or an iPSC (induced pluripotent stem cell). The musculoskeletal stem cell of the present disclosure can be easily induced from a human embryonic stem cell or a human-derived pluripotent stem cell and can be effectively differentiated not only into bone but also into cartilage, tendon and muscle. Accordingly, it can be usefully used for prevention or treatment of various musculoskeletal diseases.
Claims61
A method of preparing musculoskeletal stem cells (MSSCs), the method comprising:
- culturing cells comprising embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in a culture medium composition,
- wherein the culture medium composition comprises noggin, LIF (leukemia inhibitory factor), bFGF (basic fibroblast growth factor), Wnt signaling activator, ERK (extracellular signal-regulated kinase) signaling inhibitor and TGF-β/activin/nodal signaling inhibitor,
- wherein the cells, after the culturing, are MSSCs and exhibit the following characteristics:
- positive for nestin (NES);
- positive for Pax7;
- positive for α-SMA;
- negative for LIN28;
- and
- negative for CD90.
A musculoskeletal stem cell having the following characteristics:
- positive for nestin (NES);
- positive for Pax7;
- positive for α-SMA;
- positive for CD146;
- negative for LIN28;
- and
- negative for CD90.
Dependencies are inferred from the claim text.