Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
AssigneeThe Regents of the University of California
InventorsGuillermo C. Bazan, Brent S. Gaylord, Shu Wang
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Abstract
Methods compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
Claims18
A light harvesting multichromophore system, the system comprising
- a) a signaling chromophore;
- and
- b) a water-soluble conjugated polymer comprising a delocalized electronic structure, wherein the polymer can transfer energy from its excited state to the signaling chromophore to provide a greater than 4 fold increase in fluorescence emission from the signaling chromophore than can be achieved by direct excitation.
An aqueous composition comprising a light harvesting multichromophore system comprising (a) a signaling chromophore in solution, and (b) a water-soluble conjugated polymer comprising a delocalized electronic structure, wherein the polymer can transfer energy from its excited state to the signaling chromophore to provide a greater than 4 fold increase in fluorescence emission from the signaling chromophore than can be achieved by direct excitation.
Dependencies are inferred from the claim text.