Compositions and methods for the production of alpha-herpesviruses
AssigneeSanofi Pasteur Biologics, LLC
InventorsSimon Delagrave, Rachid Oubelaid, John Hamberger
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Abstract
The present invention relates to virus growth media that improve the yield of alpha-herpesviruses (e.g., HSV-2) grown in cell cultures. The growth media of the invention include two additives, a disaccharide and a lipid mixture, that can be added to serum-free or serum-enriched growth media to improve the efficiency of virus production. The invention further provides methods of producing alpha-herpesviruses (e.g., HSV-2) in such growth media.
Claims27
A composition comprising (i) a growth medium supplemented with between 5 mM and 500 mM of a disaccharide and a lipid mixture, (ii) Vero cells that express herpes simplex virus 2 (HSV-2) polypeptides UL5 and UL29, and (iii) an HSV-2 having inactivating mutations in UL5 and UL29.
A composition comprising (i) a growth medium supplemented with between 5 mM and 500 mM of a disaccharide and a lipid mixture, (ii) Vero cells engineered to express herpes simplex virus polypeptides UL5 and UL29, and (iii) a herpes simplex virus 2 (HSV-2) having inactivating mutations in UL5 and UL29.
Dependencies are inferred from the claim text.