Chemical amplification based on fluid partitioning
AssigneeLawrence Livermore National Security, LLC
InventorsBrian L. Anderson, Bill W. Colston, Christopher J. Elkin
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Abstract
A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.
Claims30
An apparatus for nucleic acid amplification of a sample, comprising:
- means for partitioning said sample into partitioned sections, wherein said means for partitioning said sample into partitioned sections comprises an injection orifice, and
- means for performing PCR on said partitioned sections of said sample.
A method of nucleic acid amplification of a sample, comprising the steps of:
- partitioning said sample into partitioned sections, wherein said step of partitioning said sample into partitioned sections comprises flowing said sample through an injection orifice, and
- subjecting said partitioned sections of said sample to PCR.
A method of nucleic acid amplification of a sample, comprising the steps of:
- a. partitioning the sample into partitioned sections, wherein partitioning comprises flowing the sample through an injection orifice into an immiscible carrier fluid, wherein the sample comprises a nucleic acid and components for performing nucleic acid amplification, wherein the partitioned sections comprise a gelling agent or a gel;
- and
- b. moving the partitioned sections between a first substrate and a second substrate;
- and
- c. performing nucleic acid amplification of the partitioned sections between the first substrate and the second substrate.
Dependencies are inferred from the claim text.