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US RE45,968 E1Granted
Vector for efficient selection and/or maturation of an antibody and uses thereof
AssigneeSigma-Tau Industrie Farmaceutiche Riunite S.p.A.
InventorsOlga Minenkova, Emiliano Pavoni
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Abstract
It is described a vector suitable for efficient selection and/or maturation of a recombinant antibody characterized in that it contains at least one element able to reduce the expression level and/or has an improved efficiency of display of said recombinant antibody.
Claims21
An isolated or purified vector suitable for efficient selection and affinity maturation of a recombinant antibody, the recombinant antibody having a leader peptide and being encoded by a recombinant antibody coding sequence, wherein
- the vector comprises a leader sequence encoding a leader peptide expressed with the recombinant antibody,
- the vector further comprising at least one element able to reduce the expression level of said recombinant antibody, the at least one element being selected from
- a) a suppressed stop codon inside either the leader peptide or the recombinant antibody coding sequence;
- b) a low-efficient promoter driving transcription of said recombinant antibody coding sequence;
- or
- c) an inhibitor of the promoter driving transcription of said recombinant antibody coding sequence;
- and
- wherein
- the vector comprises a further element able to provide an efficient display of said recombinant antibody, the further element comprising:
- a) a sequence coding for a carboxy-terminal part of a minor coat protein pill fused to the recombinant antibody coding sequence;
- b) a sequence coding for a leader peptide of the alkaline phosphatase of E. coli comprised as the leader peptide of the recombinant antibody sequence;
- and
- c) a coding sequence for the minor coat protein pill fused to the recombinant antibody coding sequence with no amber codon therebetween.
10 more elements
Dependencies are inferred from the claim text.