# US RE40,948 E1 · APC antibodies

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- Number: US RE40,948 E1
- Document ID: USRE40948E1
- Status: Granted
- Kind: E1
- Application number: 09/983,543
- Filed: 2001-10-24
- Granted: 2009-10-27
- Earliest claimed: 1991-01-16
- Assignees: The Johns Hopkins University; Astrazeneca United Kingdom, Ltd.; Cancer Institute, Japanese Foundation for Cancer Research; University of Utah
- Inventors: Bert Vogelstein; Kenneth W. Kinzler; Hans Albertsen; Rakesh Anand; Mary Carlson; Joanna Groden; Philip Hedge; Geoff Joslyn; Alexander Fred Markham; Yusuke Nakumura; Andrew Thliveris; Raymond White
- Web page: https://patentgrep.com/patent/USRE40948E1

## Abstract

A human gene termed APC is disclosed. Methods and kits are provided for assessing mutations of the APC gene in human tissues and body samples. APC mutations are found in familial adenomatous polyposis patients as well as in sporadic colorectal cancer patients. APC is expressed in most normal tissues. These results suggest that APC is a tumor suppressor.

## Claims

### Claim 1

A preparation of antibodies which specifically binds to a human APC (adenomatous polyposis coil) protein having an amino acid sequence as shown in SEQ ID NO:1, 2, or 7, and does not specifically bind to other human proteins.

### Claim 2

A preparation of antibodies which specifically binds to a human APC protein which is the product of a mutant allele found in a tumor, wherein the antibodies do not specifically bind to other human proteins, and wherein the human APC protein is a mutant form of the amino acid sequence shown in SEQ ID NOS:2 and SEQ ID NO: 7, and the mutant allele is a mutant form of the nucleotide sequence shown in SEQ ID NO:1 having the sequence of SEQ ID NO: 7 but for a substitution of Arg→Cys at residue 414.

### Claim 3 (depends on 2)

The preparation of claim 2 wherein the mutant allele contains a mutation selected from the group consisting of mutations at codons 243, 279, 288, 301, 331, 413, 437, 456, 500, 712, and 1338.

### Claim 4 (depends on 2)

The preparation of claim 2 wherein the mutant allele contains a premature stop codon.

### Claim 5 (depends on 2)

The preparation of claim 2 wherein the mutant allele contains a missense mutation.

### Claim 6 (depends on 2)

The preparation of claim 2 wherein the mutant allele contains a frameshift mutation.

### Claim 7 (depends on 2)

The preparation of claim 2 wherein the mutant allele contains a splice junction mutation.

### Claim 8 (depends on 2)

The preparation of claim 2 wherein the mutant allele contains an insertion mutation.