Preparation of agarose coated, solid agarose beads containing secretory cells
AssigneeThe Rogosin Institute
InventorsKanti Jain, Albert L. Rubin, Barry Smith
Life of this patent
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Abstract
Biological agents such as secretory cells are encapsulated in a hydrophilic gel made of agarose or collagen-agarose and gelatin sponge-agarose combinations. In a preferred embodiment, semi-solid beads are formed from a suspension containing collagen, agarose and secretory cells such as pancreatic islets, the collagen is polymerized to form solid, agarose-collagen beads and the solid beads are coated with agarose. Coating is preferably by rolling the solid beads in about 5-10% agarose, contacting the rolled beads with mineral oil and washing oil from the beads. Beads containing secretory cells can be transplanted into a mammal to treat a condition caused by impaired secretory cell function.
Claims49
A method for preparing an agarose coated, solid agarose-collagen bead which contains secretory cells, comprising:
- (a) suspending secretory cells in a collagen containing solution,
- (b) adding agarose to said solution,
- (c) forming a semi-solid bead of said collagen, agarose, and secretory cells,
- (d) polymerizing collagen in said semisolid bead to form a solid, agarose-collagen bead containing secretory cells, and
- (e) coating said solid, agarose-collagen bead containing secretory cells with agarose.
A composition of matter comprising a sample of secretory cells in a bead which comprises agarose, wherein said bead is coated with a solution comprising from 5% to 10% agarose.
A composition of matter comprising a sample of secretory cells entrapped in a bead which comprises from 0.5% to 5% agarose, wherein said bead is coated with a coating of agarose.
A composition of matter produced by combining a sample of secretory cells and a solution which contains from 0.5% to 5% agarose to form a suspension, treating said suspension to form a bead, and coating said bead with agarose.
A composition of matter comprising a sample of secretory cells contained in a bead which comprises agarose and collagen, wherein said bead is coated with a coating of agarose.
A method for preserving secretory cells, comprising:
- (a) forming an agarose coated, agarose containing bead, or an agarose coated bead containing both agarose and collagen, with secretory cells entrapped therein, and,
- (b) maintaining the secretory cell containing bead to preserve the secretory cells therein.
Dependencies are inferred from the claim text.