Monoclonal antibodies against human colon carcinoma-associated antigens and uses therefor
AssigneeInternational Bio-Immune Systems, Inc.
InventorsKwong Y. Tsang, Myron Arlen
Life of this patent
Estimating the term…
Abstract
Monoclonal antibodies, in particular 33.28 and 31.1, and chimeric antibodies, in particular mouse/humans chimeric Chi #1 specific for glycoprotein antigens of colon carcinoma-associated antigens which are immunogenic in humans, are disclosed. Such antibodies, and fragments and derivatives thereof, are useful in immunodiagnosis and immunotherapy of human colon, breast, and ovarian cancer, and for purification of antigens which can serve as immunotherapeutic agents. Methods of detecting the colon carcinoma-associated antigen in a sample, and methods for treating subjects having colon, breast, and ovarian carcinomas are disclosed.
Claims50
A monoclonal antibody specific for a purified human colon carcinoma-associated protein antigen, wherein said antigen has the following characteristics:
- (a) said antigen is purified to the extent that the membrane fractions are free of HL-A antigen and are substantially free from non-immunogenic glycoprotein fractions;
- (b) said antigen is not detectable on normal colon cancer free human tissues;
- (c) said antigen is not detectable on human carcinoma cells other than colon carcinoma cells;
- (d) said antigen is specifically immunogenic in humans;
- and
- (e) said antigen induces an immune response in humans having colon carcinoma which is expressed as cell mediated immunity which is murine monoclonal antibody 33.28 as produced by hybridoma PCA 33.28, deposited with the American Type Culture Collection and assigned accession number PTA-5413.
A method for diagnosing colon cancer in humans comprising:
- (a) removing a histological specimen from a patient suspected of having a colon cancer;
- (b) contacting the specimen with monoclonal antibody 33.28 (ATCC HB-12315) , as produced by hybridoma PCA 33.28, deposited with the American Type Culture Collection and assigned accession number PTA-5413;
- (c) staining the specimen with an immunohistochemical stain;
- and
- (d) detecting the presence of the antigen-antibody complex by the stain.
A method for diagnosing colon cancer in humans comprising:
- (a) removing a histological specimen from a patient suspected of having colon- carcinoma;
- (b) contacting the specimen with mouse monoclonal antibody 31.1 (ATCC HB-12314) , as produced by hybridoma PCA 31.1, deposited with the American Type Culture Collection and assigned accession number PTA-2497);
- (c) staining the specimen with an immunohistochemical stain;
- and
- (d) detecting the presence of the antigen-antibody complex.
A kit for the immunohistochemical detection of colon carcinoma comprising:
- (a) mouse monoclonal antibody 31.1 (ATCC HB-12314) , as produced by hybridoma PCA 31.1, deposited with the American Type Culture Collection and assigned accession number PTA-2497;
- (b) reagents for immunoperoxidase and secondary antibody;
- (c) immunoperoxidase;
- and
- (d) colorizing reagents.
A kit for the immunohistochemical detection of colon carcinoma comprising:
- (a) mouse monoclonal antibody 33.28 (ATCC HB-12315 , as produced by hybridoma PCA 33.28, deposited with the American Type Culture Collection and assigned accession number PTA-5413;
- (b) reagents for immunoperoxidase and secondary antibody;
- (c) immunoperoxidase;
- and
- (d) colorizing reagents.
A compartmentalized kit for the detection of a human colon carcinoma-associated antigen, wherein the antigen has the following characteristics:
- (a) said antigen is purified to the extent that the membrane fractions are free of HL-A antigen and are substantially free from non-immunogenic glycoprotein fractions;
- (b) said antigen is not detectable on normal colon cancer free human tissues;
- (c) said antigen is not detectable on human carcinoma cells other than colon carcinoma cells;
- (d) said antigen is specifically immunogenic in humans;
- and
- (e) said antigen induces an immune response in humans having colon carcinoma which is expressed as cell mediated immunity,
- said kit comprising a first container adapted to contain an antibody to said antigen or an active component thereof, and a second container adapted to contain a second antibody to said antigen or an active component thereof, said second antibody being labeled with a reporter molecule capable of giving a detectable signal.
A compartmentalized kit for the detection of a human colon carcinoma-associated antigen, wherein the antigen has the following characteristics:
- (a) said antigen is purified to the extent that the membrane fractions are free of HL-A antigen and are substantially free from non-immunogenic glycoprotein fractions;
- (b) said antigen is not detectable on normal colon cancer free human tissues;
- (c) said antigen is not detectable on human carcinoma cells other than colon carcinoma cells;
- (d) said antigen is specifically immunogenic in humans;
- and
- (e) said antigen induces an immune response in humans having colon carcinoma which is expressed as cell mediated immunity,
- said kit comprising a first container adapted to contain monoclonal antibody 31.1 (ATCC HB-12314) to said antigen and a second container adapted to contain a second antibody to said antigen or an active component thereof, said second antibody being labeled with a reporter molecule capable of giving a detectable signal.
A compartmentalized kit for the detection of a human colon carcinoma-associated antigen, wherein the antigen has the following characteristics:
- (a) said antigen is purified to the extent that the membrane fractions are free of HL-A antigen and are substantially free from non-immunogenic glycoprotein fractions;
- (b) said antigen is not detectable on normal colon cancer free human tissues;
- (c) said antigen is not detectable on human carcinoma cells other than colon carcinoma cells;
- (d) said antigen is specifically immunogenic in humans;
- and
- (e) said antigen induces an immune response in humans having colon carcinoma which is expressed as cell mediated immunity,
- said kit comprising a first container adapted to contain monoclonal antibody 33.28 (ATCC HB-12315) to said antigen and a second container adapted to contain a second antibody to said antigen or an active component thereof, said second antibody being labeled with a reporter molecule capable of giving a detectable signal.
A method for diagnosing colon cancer in humans comprising:
- (a) removing a histological specimen from a patient suspected of having a colon carcinoma;
- (b) contacting the specimen with mouse/human chimeric antibody which binds to an antigen which binds to mouse/human chimeric antibody Chi #1 (ATCC as produced by the cell line deposited with the American Type Culture Collection and assigned accession number CRL-12316) ;
- (c) staining the specimen with an immunohistochemcial stain;
- and
- (d) detecting the presence of the antigen-antibody complex by the stain.
A kit for the immunohistochemical detection of colon carcinoma comprising:
- (a) mouse/human chimeric antibody Chi #1 (ATCC CRL-12316);
- (b) reagents for immunoperoxidase and secondary antibody;
- (c) immunoperoxidase;
- and
- (d) colorizing reagents.
Dependencies are inferred from the claim text.