DNA encoding human endothelial cell growth factors and plasmids comprising said DNA
AssigneeAventis Pharmaceuticals Inc.
InventorsMichael C. Jaye, Wilson Burgess, Thomas Maciag, William N. Drohan
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Abstract
The present invention is directed to DNA encoding human endothelial cell growth factors, and to plasmids comprising said DNA. In particular, the invention relates to DNA encoding a cleavable signal peptide and an endothelial cell growth factor, wherein removal of said signal peptide yields a mature form of the growth factor.
Claims29
An isolated DNA encoding a cleavable signal peptide and an endothelial cell growth factor, wherein removal of said signal peptide yields a mature form of said endothelial cell growth factor, and said endothelial cell growth factor either has the amino acid sequence of α-endothelial cell growth factor
- (NYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHI QLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQ TPNEECLFLERLEENHYNTYI S K K H A E K N W F V G L K KNGSCKRGPRTHYGQKAILFLPLPVSSD) or comprises the amino acid sequence of α-endothelial β-βendothelial cell growth factor
- (AEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLR ILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETG QYLAMDTDGLYYGSQTPNEECLFLERLEENHYNTYIS KKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPL PVSSD).
A DNA encoding a cleavable heterologous signal peptide and an endothelial cell growth factor, wherein removal of said signal peptide yields a mature form of said endothelial cell growth factor, and said endothelial cell growth factor either has the amino acid sequence of α-endothelial cell growth factor
- (NYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHI QLQLSAEVSVGEVYIKSTETGQYLAMDTDGLLYGSQ TPNEECLFLERLEENHYNTY I S K K H A E K N W F V G L K KNGSCKRGPRTHYCQKAILFLPLPVSSD) or comprises the amino acid sequence of β-endothelial cell growth factor
- (AEGEITTFTALTAKFNLPPGNYKKPKLLYCSNGGHFLR ILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETG QYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYIS KKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPL PVSSD).
A plasmid comprising a DNA encoding a cleavable signal peptide and an endothelial cell growth factor, wherein removal of said signal peptide yields a mature form of said endothelial cell growth factor, and said endothelial cell growth factor either has the amino acid sequence of α-endothelial cell growth factor
- (NYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHI QLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQ TPNEECLFLERLEENHYNTY I S K K H A E K N W F V G L K KNGSCKRGPRTHYGQKAILFLPLPVSSD)
- or comprises the amino acid sequence of β-endothelial cell growth factor
- (AEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLR ILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETG QYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYIS KKHAQEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPL PVSSD).
A plasmid comprising a DNA encoding a cleavable heterologous signal peptide and human α-endothelial cell growth factor comprising having the amino acid sequence
- N Y K K K L L Y C S N G G H F L R I L P DGTVDGTRDRSDQHI QLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQ TPNEECLFLERLEENHYNTYISKKHAEKNWFVGLK KNGSCKRGRRTHYGQKAILFLPLPVSSD,
- wherein removal of said signal peptide yields a mature form of said human α-endothelial cell growth factor.
A plasmid comprising a DNA encoding a cleavable heterologous signal peptide and human β-endothelial cell growth factor comprising the amino acid sequence
- AEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLR ILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETG QYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYIS KKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPL PVSSD,
- wherein removal of said signal peptide yields a mature form of said human β-endothelial cell growth factor.
Dependencies are inferred from the claim text.