Nucleotide sequences of canola and soybean palmitoyl-ACP thioesterase genes and their use in the regulation of fatty acid content of the oils of soybean and canola plants
AssigneeE. I. Du Pont de Nemours and Company
InventorWilliam Dean Hitz
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Abstract
The preparation and use of nucleic acid fragments encoding acyl-acyl carrier protein thioesterase enzymes to modify plant lipid composition are disclosed. Also disclosed are chimeric genes incorporating such nucleic acid fragments and suitable regulatory sequences may be used to create transgenic plants with altered levels of saturated fatty acids.
Claims34
An isolated nucleic acid fragment encoding a plant acyl-ACP thioesterase wherein said thioesterase has a preference of at least two-fold for palmitoyl-ACP over either stearoyl-ACP or oleoyl-ACP and farther wherein said isolated nucleic acid fragment hybridizes to one of the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 31 under the following set of conditions:
- hybridization at 60° C. in 6×SCC, 0.1% SDS for 18 hr, washing at 60° C. in 0.2×SSC, 0.1% SDS twice for 10 min each.
An isolated nucleic acid fragment encoding the soybean seed acyl-ACP thioesterase encoded by nucleotides 1 to 1688 of SEQ ID NO:1.
An isolated nucleic acid fragment encoding the canola seed acyl-ACP thioesterase encoded by nucleotides 1 to 1483 of SEQ ID NO:2.
An isolated nucleic acid fragment encoding the canola seed acyl-ACP thioesterase encoded by nucleotides 1 to 1674 of SEQ ID NO:31.
An isolated nucleic acid fragment encoding a plant acyl-ACP thioesterase wherein said thioesterase has a preference of at least two-fold for palmitoyl-ACP over either stearoyl-ACP or oleoyl-ACP and further wherein said isolated nucleic acid fragment hybridizes to nucleotides 506 to 1477 of SEQ ID NO:1, nucleotides 255 to 1226 of SEQ ID NO:2, or nucleotides 479 to 1438 of SEQ ID NO:31 under the following set of conditions:
- hybridization at 60° C. in 6×SCC, 0.1% SDS for 18 hr, washing at 60° C. in 0.2×SSC, 0.1% SDS twice for 10 min each.
An isolated nucleic-acid fragment encoding a soybean acyl-ACP thioesterase having the amino acid sequence of SEQ ID NO:29.
An isolated nucleic-acid fragment encoding a rapeseed acyl-ACP thioesterase having the amino acid sequence of SEQ ID NO:30.
An isolated nucleic-acid fragment encoding a rapeseed acyl-ACP thioesterase having the amino acid sequence of SEQ ID NO:32.
Dependencies are inferred from the claim text.