Protein separations by capillary electrophoresis using amino acid-containing buffers
AssigneeBio-Rad Laboratories, Inc.
InventorsEli Grushka, Cheng-Ming Liu
Life of this patent
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Abstract
Protein mixtures are separated by capillary electrophoresis in a buffer containing one or more amino acids in an amount sufficient to prevent or substantially reduce the degree of protein binding to the wall of the capillary.
Claims18
A method of separating a mixture of proteins in a liquid sample, said method comprising electrophoretically passing said sample through a capillary filled with an alkaline solution of an amino acid at a sufficient concentration to be effective as a buffering agent, and in which said amino acid is the sole buffering agent in said alkaline solution , by imposing an electric field across said capillary, wherein said capillary has an inner surface and said inner surface is uncoated.
Dependencies are inferred from the claim text.