Process for the enzymatic determination of glucose with a glucose-oxydazed/peroxidazed enzyme system
AssigneeIstituto Sieroterapico e Vaccinogeno Toscano "Sclavo" S.p.A.
InventorFranco Meiattini
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Abstract
Process for enzymatic determination of glucose with a glucose-oxidase-peroxidase enzyme system involving the use of a reduced acceptor which is oxidized. As reduced acceptor in the enzymatic reaction catalyzed by peroxidase, a ferrocyanide salt is oxidized to ferricyanide; then a further reducing system is introduced, which system is oxidized by the ferricyanide, with the formation of a colored quinone compound, the reaction between ferricyanide and the reducing system taking place spontaneously.
Claims17
A process for the enzymatic determination of the amount of glucose present in body fluids which comprises the steps:
- i. adding to a sample of a body fluid an enzymatic system containing glucose-oxidase and peroxidase, to which has been added sodium or potassium ferrocyanide,
- ii. incubating the sample and enzyme system at room temperature whereby the ferrocyanide is converted to ferricyanide equivalent to the amount of glucose present in the sample, and
- iii. reacting the ferricyanide with a reducing system which forms a colored product on oxidation, the color being a measure of the ferricyanide present, said reducing system consisting of a phenolic compound and an aminoantipyrine.
A process for the enzymatic determination of the amount of glucose in an undeproteinized body fluid which consists in adding to said undeproteinized body fluid an aqueous solution of an enzymatic system consisting of glucose-oxidase and peroxidase, an aminoantipyrine and a phenolic compound which is a member of the group consisting of salicylamide, barbituric acid, para- and meta-hydroxy-benzoate and esters thereof, and thereafter measuring color change photometrically.
A composition for use in the enzymatic determination of the amount of glucose in a body fluid, consisting essentially of a glucose-oxidase and peroxidase enzyme system, a phenolic compound selected from the group consisting of salicylamide, barbituric acid, para- and meta-hydroxybenzoate and esters thereof, and an aminoantipyrine.
Dependencies are inferred from the claim text.