Method for determining vitamin B.sub.12 and reagent therefor
AssigneePharmacia Corporation
InventorsRolf E. A. V. Axen, Jerker O. Porath, Leif Edvin Wide
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Abstract
Method for determining vitamin B.sub.12 in aqueous samples by contacting said sample with radioactivity labelled vitamin B.sub.12 and with particles of water insoluble polymers to which substances capable of binding vitamin B.sub.12 have been bound, thereafter separating said particles and determining the radioactivity.
Claims31
A method for determining vitamin B.sub.12 in an aqueous sample, which comprises contacting particles of water-insoluble polymers to which a substance capable of binding vitamin B.sub.12 has been bound, by covalent bonds, with the sample and with a certain amount of vitamin B.sub.12 labelled with a radioisotope, and subsequent to the reaction between vitamin B.sub.12 and the substance capable of binding vitamin B.sub.12 attached to the particles having taken place, separating the particles from the sample liquid and determining the radioactivity of the particle material.
A method for determining vitamin B.sub.12 in an aqueous sample, which comprises contacting particles of water-insoluble polymers to which a substance capable of binding vitamin B.sub.12 has been bound, by covalent bonds, with the sample and with a certain amount of vitamin B.sub.12 labelled with a radioisotope, and subsequent to the reaction between vitamin B.sub.12 and the substance capable of binding vitamin B.sub.12 attached to the particles having taken place, separating the particles from the sample liquid and determining the radioactivity in the fluid.
A reagent for use in the determination of vitamin B.sub.12, containing particles of water insoluble polymers to which have been bound, by covalent bonds, a substance capable of binding vitamin B.sub.12, in dried form.
Test pack for the determination of vitamin B.sub.12 chiefly comprising one or more sealed ampoules containing particles of water insoluble polymers to which have been bound, by covalent bonds, a substance capable of binding vitamin B.sub.12 in dried form and another ampoule with vitamin B.sub.12 labelled with a radioisotope in dried form.
Dependencies are inferred from the claim text.