Method for the determination of proteins and polypeptides
AssigneePharmacia Corporation
InventorsRolf E. A. V. Axen, Jerker O. Porath, Leif Edvin Wide
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Abstract
A process comprising contacting particles of water insoluble polymers to which have been bound antibodies, by means of covalent bonds, against the protein or polypeptide to be determined, wherein a certain quantity of the protein or polypeptide is labeled with a radioactive isotope, whereupon the particles, subsequent to the reaction between the protein or the polypeptide and the antibodies attached to the particles are separated from the sample liquid and the radioactivity of the particle material and/or in the liquid is determined.
Claims23
A method for the determination of a member selected from the group consisting of proteins and polypeptides in aqueous samples, which comprises contacting particles of water insoluble polymers to which have been bound, by covalent bonds, antibodies against the said member to be determined, with the aqueous sample containing said member and with the same member labelled with a radioactive isotope to bind part of said labelled member and unlabelled member to said antibodies to produce a two-phase system comprising a solid phase comprising said bound part of labelled member and unlabelled member and a liquid phase comprising unbound labelled member and unlabelled member, separating the two phases from each other, and measuring the radioactivity of at least one of the said solid and said liquid phases, the value of said radioactivity being each a function of the concentration of the said member in the aqueous sample.
A method as claimed 1, wherein the determination is effected quantitatively by comparing the measured value of the radioactivity with a standard curve.
Dependencies are inferred from the claim text.