Streptococcus pneumoniae gene HI0454
AssigneeEli Lilly and Company
InventorsRobert Brown Peery, Paul Luther Skatrud, Michele Louise Young Bellido, Patti Jean Treadway
Life of this patent
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Abstract
The invention provides isolated nucleic acid compounds encoding the 454 gene of Streptococcus pneumoniae. Also provided are vectors and transformed host cells for expressing the encoded protein, and a method for identifying compounds that bind and/or inhibit said protein.
Claims26
An isolated nucleic acid fragment encoding a protein having the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid fragment, wherein said fragment has a sequence selected from the group consisting of:
- (a) SEQ ID NO:
- 1;
- (b) SEQ ID NO:
- 3;
- (c) a nucleic acid fragment that encodes the same protein as (a) or (b), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nucleic acid fragment fully complementary to (a), (b), or (c).
An isolated nucleic acid fragment, wherein said fragment has a sequence specified herein as SEQ ID NO:4.
An isolated nucleic acid fragment that hybridizes to the complementary strand of SEQ ID NO:
- 1 or SEQ ID NO:3 under high stringency conditions comprising hybridizing at 50° C. to 65° C. in 5×SSPE and 50% formamide, and washing at 50° C. to 65° C. in 0.5×SSPE, and that encodes a protein having the ligand binding activity of Streptococcus pneumoniae 454 protein.
An isolated nucleic acid fragment that hybridizes to the complementary strand of SEQ ID NO:
- 1 or SEQ ID NO:3 under low stringency conditions comprising hybridizing at 35° C. to 37° C. in 5×SSPE and 40% to 45% formamide, and washing at 42° C. in 1×-2×SSPE, and that encodes a protein having the ligand binding activity of Streptococcus pneumoniae 454 protein.
An isolated nucleic acid fragment consisting essentially of a nucleotide sequence encoding a protein having the amino acid sequence that is SEQ ID NO:2.
An isolated nucleic acid fragment, wherein said fragment consists essentially of a sequence selected from the group consisting of:
- (a) SEQ ID NO:
- 1;
- (b) SEQ ID NO:
- 3;
- (c) a nucleic acid fragment that encodes the same protein as (a) or (b), but which is degenerate in accordance with the degeneracy of the genetic code;
- and
- (d) a nucleic acid fragment fully complementary to (a), (b), or (c).
An isolated nucleic acid fragment, wherein said fragment consists essentially of SEQ ID NO:4.
Dependencies are inferred from the claim text.