Thermophilic DNA polymerase
OwnerIlse I. Blumentals, Roger S. Lasken +4inventors
InventorsIlse I. Blumentals, Roger S. Lasken, Brian J. Schmidt, Mary C. Longo, A. John Hughes, Jr., Deb K. Chatterjee
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Abstract
The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.
Claims2
A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2.
A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2 except wherein asparagine at position 141 has been replaced by alanine and glutamine at position 143 has been replaced by alanine.
Dependencies are inferred from the claim text.