Construction of mitochondrial UQCRB mutant expressing cells and utilization of the cells for UQCRB assay system thereof

A method for angiogenesis inhibitory activity evaluation, the method comprising:

• (a) establishing a mitochondrial ubiquinol-cytochrome c reductase binding protein (UQCRB) mutant animal cell line expressing a UQCRB mutant protein, wherein the mitochondrial UQCRB mutant animal cell line is transfected with a vector including a nucleotide sequence coding human ubiquinol-cytochrome c reductase binding protein (UQCRB) mutant of SEQ ID NO:
• 1, to express the human UQCRB mutant protein;
• (b) contacting a test substance with the animal cells;
• and
• (c) analyzing the angiogenesis inhibitory activity of the test substance.

A method for screening a UQCRB activity inhibitor, the method comprising:

• (a) establishing a mitochondrial ubiquinol-cytochrome c reductase binding protein (UQCRB) mutant animal cell line expressing a UQCRB mutant protein, wherein the mitochondrial UQCRB mutant animal cell line is transfected with a vector including a nucleotide sequence coding human ubiquinol-cytochrome c reductase binding protein (UQCRB) mutant of SEQ ID NO:
• 1, to express the human UQCRB mutant protein;
• (b) contacting a test substance with the animal cells;
• and
• (c) analyzing the UQCRB inhibitory activity of the test substance.