Hypoxia Inducible Factor (HIF) Activity Reporter Cell Line
AssigneeUniversiti Putra Malaysia
InventorsNorazizah Shafee, Eric J. Stanbridge, Khatijah Yusoff, Sien-Yei Liew
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Abstract
The present invention relates to the construction of a new hypoxia inducible factor (HIF) responsive reporter gene construct, the genetic constructs and vectors containing the same. Further, the present invention relates to a stable cell line comprising the HIF responsive reporter construct, as well as methods and uses of the inventive constructs and cell lines to identify modulators of HIF activity.
Claims4
A Saos-2 cell line that comprises stably integrated into its genome a gene construct consisting of a TATA box and a reporter gene integrated under the control of a hypoxia inducible factor (HIF) responsive promoter, wherein the HIF responsive promoter consists of four hypoxia-response elements (HREs) of the human erythropoietin (EPO) gene located in a tandem repeat, and wherein the HIF responsive promoter is upstream of the reporter gene.
A gene construct consisting of a TATA box and a reporter gene under the control of a hypoxia inducible factor (HIF) responsive promoter, wherein the HIF responsive promoter is at most 100 base pairs long and consists of four hypoxia-responsive elements (HREs) of the human erythropoietin (EPO) gene located in a tandem repeat;
- and
- wherein the HIF responsive promoter is upstream of the reporter gene.
Dependencies are inferred from the claim text.