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US 9,994,883 B2Granted
Triterpenoid sapogenin production in plant and microbial cultures
AssigneesVib Vzw, Universiteit Gent
InventorsAlain Goossens, Tessa Moses, Jacob Pollier, Lorena Almagro Romero
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Abstract
The disclosure relates to a method for enhancing the biosynthesis and/or secretion of sapogenins in the culture medium of plant and microbial cell cultures. Further, the disclosure also relates to the identification of novel genes involved in the biosynthesis of sapogenin intermediates, as well as to novel sapogenin compounds.
Claims11
A method for the intracellular production and extracellular secretion of triterpenoid sapogenins in a genetically engineered yeast culture, the method comprising:
- incubating genetically engineered yeast cells that express an exogenous regulatory or biosynthetic enzyme of the sapogenin biosynthesis pathway in a culture medium comprising randomly methylated cyclodextrins, hydroxypropylated cyclodextrins, and/or beta-cyclodextrins at a concentration such that the cyclodextrins increase the amount of the produced triterpenoid sapogenin secreted from the eukaryotic genetically engineered yeast cells into the culture medium in comparison to a culture medium lacking said cyclodextrins where the biosynthetic enzyme is an oxidosqualane cyclase and/or a cytochrome P450.
Dependencies are inferred from the claim text.