Recombinant microorganisms and uses therefor
AssigneeLanzatech New Zealand Limited
InventorsMichael Koepke, Wendy Yiting Chen
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Abstract
Bacteria are genetically engineered to produce 3-hydroxypropionate (3-HP). The bacteria are carboxydotrophic acetogens. The bacteria produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/CO2. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.
Claims13
A recombinant carboxydotrophic, acetogenic bacterium comprising two gas-fixing fermentation pathways, including a Wood-Ljungdahl pathway capable of converting 2 molecules of CO or CO2 into one molecule of acetyl-CoA and a 3-hydroxypropionate biosynthesis pathway capable of converting 1 molecule of CO2 and 1 molecule of acetyl-CoA into 1 molecule of 3-hydroxypropionate, wherein the 3-hydroxypropionate biosynthesis pathway comprises an acetyl-CoA carboxylase (EC 6.4.1.2) and a malonyl-CoA reductase (EC 1.2.1.75), wherein at least the malonyl-CoA reductase is exogenous to the bacterium, and wherein the bacterium is derived from a parental bacterium selected from the group consisting of Clostridium autoethanogenum, Clostridium ragsdalei, Clostridium carboxidivorans, Clostridium drakei, Clostridium scatologenes, Clostridium aceticum, Clostridium formicoaceticum, Clostridium magnum, Butyribacterium methylotrophicum, Acetobacterium woodii, Alkalibaculum bacchi, Blautia producta, Eubacterium limosum, Moorella thermoacetica, Moorella thermoautotrophica, Sporomusa ovata, Sporomusa silvacetica, Sporomusa sphaeroides, Oxobacter pfennigii, and Thermoanaerobacter kivui.
Dependencies are inferred from the claim text.