Engineering microbes and metabolic pathways for the production of ethylene glycol
AssigneeMassachusetts Institute of Technology
InventorsGregory Stephanopoulos, Brian Pereira, Marjan De Mey, Deepak Dugar, Jose Luis Avalos
Life of this patent
Estimating the term…
Abstract
The invention relates to recombinant cells and their use in the production of ethylene glycol.
Claims26
A bacterial cell engineered to produce glycolaldehyde from xylose, wherein the cell has reduced or eliminated activity of, or reduced or eliminated expression of, xylulokinase relative to a wild-type cell, the cell has reduced or eliminated activity or reduced or eliminated expression of aldehyde dehydrogenase A relative to a wild-type cell, and the cell recombinantly expresses an enzyme that interconverts xylulose and ribulose.
An Escherichia coli cell engineered to produce glycolaldehyde, wherein the cell comprises a deletion of a xylB gene and recombinantly expresses a dte gene obtained from Pseudomonas cichorii, a fucK gene obtained from Escherichia coli, and a fucA gene obtained from Escherichia coli.
An Escherichia coli cell engineered to produce ethylene glycol, wherein the cell comprises a deletion of a xylB gene and an aldA gene, and recombinantly expresses a dte gene obtained from Pseudomonas cichorii, a fucK gene obtained from Escherichia coli, a fucA gene obtained from Escherichia coli, and a fucO gene obtained from Escherichia coli.
An engineered bacterial cell that comprises a deletion of a gene encoding xylulokinase and a deletion of a gene encoding aldehyde dehydrogenase A.
Dependencies are inferred from the claim text.