Rust resistance gene
AssigneesCommonwealth Scientific and Industrial Research Organisation, Grains Research and Development Corporation
InventorsEvans Lagudah, John Wallace Moore
Life of this patent
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Abstract
The present invention relates to new transporter polypeptides, and genes encoding therefor, which can be used to confer upon a plant resistance to one or more biotrophic fungal pathogens.
Claims27
A transgenic plant which has integrated into its genome a transgene comprising an exogenous polynucleotide encoding an Lr67 polypeptide which comprises amino acids having a sequence at least 95% identical to the amino acid sequence of SEQ ID NO:1, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:1, and wherein the polynucleotide is operably linked to a promoter capable of directing expression of the polynucleotide in a cell of the plant.
Flour or wholemeal comprising a transgene comprising an exogenous polynucleotide encoding an Lr67 polypeptide which comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:
- 1, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:
- 1.
A chimeric vector comprising a polynucleotide which encodes an Lr67 polypeptide which comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:
- 1, wherein the Lr67 polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:
- 1, and wherein the polynucleotide is operably linked to a promoter.
A process for determining whether a polypeptide confers resistance or susceptibility to one or more biotrophic fungal pathogen(s), comprising:
- i) obtaining a polynucleotide operably linked to a promoter, the polynucleotide encoding the polypeptide, wherein the polypeptide comprises an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:
- 1, wherein the polypeptide does not comprise a glycine at a position corresponding to amino acid number 144 of SEQ ID NO:
- 1,
- ii) introducing the polynucleotide into a plant,
- iii) determining whether the level of resistance or susceptibility to the one or more biotrophic fungal pathogen(s) is increased or decreased relative to an isogenic plant lacking the polynucleotide, and
- iv) optionally, if the level of resistance or susceptibility is increased, selecting a polynucleotide encoding the polypeptide which when expressed confers resistance or susceptibility to the one or more biotrophic fungal pathogen(s).
Dependencies are inferred from the claim text.