Production of omega 3 long chain polyunsaturated fatty acids in oilseed crops by a thraustochytrid PUFA synthase
AssigneesDow AgroSciences LLC, Dsm IP Assets B.V.
InventorsTerence A. Walsh, Daniel J. Gachotte, Cory M. Larsen, Scott Bevan, P. Ann Owens-Merlo, James G. Metz, Ross Zirkle
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Abstract
This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.
Claims38
A genetically modified Brassica plant that produces oil comprising eicosapentaenoic acid (C20:5, n-3) (EPA) in amount between 0.01% and 10% by weight of total fatty acids, the plant comprising:
- a first polynucleotide encoding a first polypeptide of a polyunsaturated fatty acid (PUFA) synthase system comprising SEQ ID NO:1;
- a second polynucleotide encoding a second polypeptide of the PUFA system comprising SEQ ID NO:4;
- a third polynucleotide encoding a third polypeptide of the PUFA system consisting of SEQ ID NO:14;
- and
- a fourth polynucleotide encoding a phosphopantetheinyl transferase (PPTase) from a Nostoc sp.,
- wherein each of the polynucleotides is operably linked to a promoter functional in the plant.
An isolated nucleic acid molecule comprising:
- a first polynucleotide encoding a first polypeptide of a polyunsaturated fatty acid (PUFA) synthase system comprising SEQ ID NO:1;
- a second polynucleotide encoding a second polypeptide of the PUFA system comprising SEQ ID NO:4;
- a third polynucleotide encoding a third polypeptide of the PUFA system consisting of SEQ ID NO:14;
- and
- a fourth polynucleotide encoding the phosphopantetheinyl transferase (PPTase) from a Nostoc sp.,
- wherein each of the polynucleotides is operably linked to a promoter functional in the plant.
A system for producing a genetically modified plant, the system comprising at least one isolated nucleic acid molecules, the system comprising:
- a first polynucleotide encoding a first polypeptide of a polyunsaturated fatty acid (PUFA) synthase system comprising SEQ ID NO:1;
- A second polynucleotide encoding a second polypeptide of the PUFA system comprising SEQ ID NO:4;
- A third polynucleotide encoding a third polypeptide of the PUFA system consisting of SEQ ID NO:14;
- and
- a fourth polynucleotide encoding a phosphopantetheinyl transferase (PPTase) from a Nostoc sp.
Dependencies are inferred from the claim text.