Method for cellular restoration

A method of restoring adult stem cells comprising:

• obtaining a donor composition from a donor subject wherein the donor composition comprises a plurality of adult stem cell types;
• obtaining a receiver composition from a receiver subject wherein the receiver composition comprises a plurality of adult stem cell types and wherein the donor subject and receiver subject differ in chronological age by from about 5 years to about 75 years;
• culturing the donor composition in a media for a time period ranging from about 1 hour to about 90 days wherein culturing the donor composition results in the production of secreted vesicles, microvesicles, cell-derived soluble vesicle compartments, cell-derived soluble factors, or a combination thereof;
• separating the adult stem cell types in the donor composition from the media to produce a cell-free donor composition wherein separating comprises filtering the cells in the donor composition through a permeable membrane;
• and
• co-culturing the cell-free donor composition and receiver composition wherein co-culturing comprises contacting the receiver composition with the cell-free donor composition to produce a restored composition wherein the restored composition comprises cells that display an altered expression of at least one gene selected from the group consisting of GPX-1 (encoding for glutathione peroxidase-1), SOD 1 (encoding for superoxide dismutase-1);
• REL-A (encoding for p65 of the NFKB heterodimer);
• LIG-4 (encoding for DNA Ligase-4);
• GLB-1 (encoding for —galactosidase) and p16INK4 and wherein the altered expression comprises a change in the expression level of the at least one gene of at least 1.5 fold.