US 9,989,531 B2 · Composition comprising a cell sample from a subject with scoliosis and a reagent for detecting PTPμ or PIPK1y

Abstract

Methods of stratifying a subject having or at risk for developing adolescent idiopathic scoliosis (AIS) into diagnostically or clinically useful subclasses are provided. The stratification is based on the subject's PTPμ expression and/or activity and/or PIPK1γ expression and/or activity. Also provided are methods of predicting the risk of developing a scoliosis also based on the subject's PTPμ expression and/or activity and/or PIPK1γ expression and/or activity; and methods of increasing GiPCR signaling in cells of a subject in need thereof comprising administering to the subject's cells an effective amount of an inhibitor of PIPK1γ tyrosine phosphorylation; an activator of PIPK1Y tyrosine dephosphorylation; and/or an inhibitor of PIPK1γ expression and/or activity.

Claims22

Dependent claims

A composition comprising:

(A) a cell sample from a subject having scoliosis or a subject which is a likely candidate for developing a scoliosis;
and
(i) at least one reagent for detecting Protein Tyrosine Phosphatase μ PTPμ expression and/or activity in the cell sample, wherein the at least one reagent for detecting PTPμ comprises (a) a non-natural oligonucleotide which hybridizes to PTPμ mRNA and which comprises a label and/or a modified base;
or (b) a PTPμ antibody;
and/or (ii) at least one reagent for detecting type I Phosphatidylinositol Phosphate Kinase isoform gamma PIPK1γ expression and/or activity in the cell sample, wherein at least one reagent for detecting PIPK1γ comprises a non-natural oligonucleotide which hybridizes to PIPK1γ mRNA and which comprises a label and/or a modified base;
or (b) a PIPK1γ antibody.

3 more elements

A method of stratifying a subject having a scoliosis or a subject which is a likely candidate for developing scoliosis, or of predicting the risk of developing a scoliosis in said subject said method comprising:

(i) providing a cell sample isolated from the subject;
(ii) (a) detecting Protein Tyrosine Phosphatase μ PTPμ expression and/or activity in the cell sample by contacting the cell sample with (a1) a non-natural oligonucleotide which hybridizes to PTPμ mRNA and which comprises a label and/or a modified base;
or (a2) a PTPμ antibody;
(b) detecting type I Phosphatidylinositol Phosphate Kinase isoform gamma (PIPK1γ) expression and/or activity in the cell sample by contacting the cell sample with (b1) a non-natural oligonucleotide which hybridizes to PIPK1γ mRNA and which comprises a label and/or a modified base;
or (b2) a PIPK1γ antibody;
or
(c) a combination of (a) and (b);
and
(iii) (a) stratifying said subject into a AIS subclass based on the level of expression or activity of PTPμ and/or PIPK1γ in the cell sample of the subject;
or
(b) determining that the subject is at risk of developing a scoliosis when:
(1) PTPμ expression and/or activity is decreased;
and/or
(2) PIPK1γ expression and/or activity is increased;
in the subject's sample as compared to a level in a control sample.

12 more elements

Dependencies are inferred from the claim text.

Composition comprising a cell sample from a subject with scoliosis and a reagent for detecting PTPμ or PIPK1y

Life of this patent

Composition comprising a cell sample from a subject with scoliosis and a reagent for detecting PTPμ or PIPK1y

Life of this patent

Abstract

Claims22